In those circumstances I would have manually set ISO to 400 and taken a series of shots and chosen the best. It is such an attractive little creature that it would have been worth the effort. Very close photographic work with small subjects is technically very challenging, and it often requires several attempts to get it right. Your aperture selection looks good, but the focus is definitely off and at such close range the DOF is very small. It's so much nicer, though, when you finally get the result you wanted. Alternatively, you have another critique that states "good detail and sharpness" so maybe my eyesight is woefully defective ;-)
Best wishes, as always, Nigel.
Phrygian oddity (3)